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Sankara Nethralaya
Sankara Nethralaya
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Profile of Sankara Nethralaya, Chennai List of Laboratory Tests offered in SN Kolkata
 
Aditya Birla Sankara Nethralaya Offers Laboratory tests on Clinical Microbiology, Serology , Molecular Microbiology,Clinical Genetics & Molecular Microbiology
 
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BACTERIOLOGY, MYCOLOGY, VIROLOGY
Gram’s stain
KOH / Calcoflour Stain
Giemsa Stain
AFB Stain
Bacterial Culture (Aerobic & Anaerobic bacteria)
Mycobacterial culture (BACTEC MICRO MGIT) method
Antibiotic(First line drugs) sensitivity for
M. tuberculosis (BACTEC Method-SHREZ)
Fungal Culture
SEROLOGY
Non Treponemal (RPR) and Treponemal (TPHA)
Rheumatoid Arthritis (RA Factor)
Anti-streptolysin O (ASO)
C- Reactive Protein (CRP)
Widal test
HBsAg (ELISA)
Antibodies to HIV 1 & HIV 2 (ELISA)
Antibodies to HCV (ELISA)
Abs. to Toxoplasma gondii - IgG & IgM (ELISA)
Abs. to Rubella Virus – IgG & IgM (ELISA)
Abs. to Cytomegalovirus - IgG & IgM (ELISA)
Abs. to Herpes Simplex Virus– IgG & IgM (ELISA)
Abs. to Epstein Barr Virus- Viral Capsid Antigen (EBV-VCA) – IgG & IgM (ELISA)
MOLECULAR MICROBIOLOGY POLYMERASE CHAIN REACTION (PCR) TESTS
Eubacterial genome
Panfungal genome
Propionibacterium acnes
Mycobacterium tuberculosis
Cytomegalovirus (CMV)
Herpes Simplex Virus (HSV) type 1 & 2
Toxoplasma gondii (T. gondii)
Chlamydia trachomatis
Chlamydia pneumoniae
Rubella Virus (by RT PCR)
Adenovirus
Varicella Zoster Virus (VZV)
HLA-B27
REAL TIME PCR TESTS
Mycobacterium tuberculosis
Human Immunodeficiency Virus
Chikungunya Virus
Human Cytomegalovirus (CMV)
Hepatitis B Virus (HBV)
Hepatitis C Virus (HCV)
Herpes Simplex Virus (HSV)
Epstein Barr virus (EBV)
BK Virus
(i) Would you like to receive a booklet on PCR tests

(ii) Please Contact: 033 64990882

(iii) The laboratory would make available the test reports within 24 hours and communicate the same to you by Telephone/Fax/email. The “lab would contact you by Telephone/Fax/email as soon as the reports are ready. To reach the lab, dial 033 64990882
COLLECTION AND TRANSPORT OF SPECIMENS
FOR POLYMERASE CHAIN REACTION (PCR) AND REAL TIME PCR

Blood: 2ml of venous blood is collected in a EDTA vacutainer, stored at 4°C and transported in ice to the laboratory with in 24 hours of collection.

Body fluids, respiratory specimens, biopsy, FNAB, pus etc: All the specimens for PCR are collected in a sterile, DNAse and RNAse free container( Can be collected from the laboratory as and when required), stored at 4°C and transported in ice to the laboratory with in 24 hours of collection.

Sections from Tissue blocks: Sections from the Biopsy specimens processed and fixed in wax (for histopathology) can be utilized for PCR for Mycobacterium tuberculosis. A set of 3- 4 slides with 3 – 4 section per slide are needed. Note: DNAse and RNAse free specimen collection containers for PCR/RTPCR can be collected from the laboratory as and when required.

CLINICAL MICROBIOLOGY

Urine for pyogenic bacteria: Urine to be collected as per standard collection procedure and transported to the laboratory with in one hour of collection

CSF: To collect in a sterile container and to be left at 37°C until transport. To be transported at room temperature to the laboratory with in 1-2 hours

Other Body fluids, respiratory specimens, biopsy, FNAB , pus etc: To collect in a sterile container and to be kept at 4°C and transported to the laboratory in cold within 2 hours of collection for bacterial culture and within 24 hours of collection for Mycobacteria and fungal culture

Sputum for Mycobacteria (AFB ) culture: Early morning first sample of sputum produced through a deep throated cough to be collected in a sterile wide mouth container and transported at room temperature within 2 hours to the laboratory. It is recommended that the test be repeated on sputum collected on two consecutive days.

Urine for Mycobacteria (AFB) culture: Early morning first voided urine sample to be collected in a sterile 500ml capacity bottle and transported in cold with in 2 hours. It is recommended that the test be repeated on urine collected on two consecutive days

Gastric lavage for Mycobacteria (AFB ) culture: The specimen to be collected in a sterile container and transported within one hour to the laboratory at room temperature as the acid fast bacilli may be killed in the acidic nature of the specimen

Swabs ( pus, urethral swab, throat swab, swab from lesion or vesicle):
The material collected in two- three sterile swabs, put in a sterile test tube with a cotton plug, stored at 4°C and transported in cold within 2 hours for bacterial culture and within 24 hours for Mycobacteria and fungal culture

Skin scraping for AFB The skin scraping can be collected from the periphery of the lesion with the blunt edge of a sterile blade.The smear to be made on a new clean microscopic slide.and fixed in methanol or acetone, transported to the laboratory at room temperature with in 24 hours of collection preferably in slide mailer or wrapped in a clean tissue paper.

Skin scraping for Fungus: The skin scraping can be collected from the periphery of the lesion with the blunt edge of a sterile blade, and placed in the envelope provided by the laboratory. The closed envelope can be transported to the laboratory at room temperature with in 24 hours

SEROLOGY
Blood: 2-3ml of venous blood collected in a vacutainer, stored at 4°C and transported in cold to the laboratory
CSF: 0.5 – 1.0 ml collected in a sterile container and transported in cold to the laboratory
CLINICAL GENETICS
Chromosomal study
MOLECULAR BIOLOGY
Rhodopsin gene screening
RPE65 gene screening
Pax6 gene screening for aniridia
Retinoschisis gene screening
CYP1B1 gene screening for Glaucoma
MYOC gene screening for open angle Glaucoma
Genetics & Molecular Biology:
Blood: 10 ml of venous blood is collected in a heparinised vial, stored at 4°C and transported at room temperature to the laboratory with in 24 hours of collection.
CLINICAL HEMATOLOGY
Haemoglobin(Hb) and Haematocrit
Total RBC count
Total WBC count/Differential count
ESR
Platelet count
Blood group& Rh type
Prothrobin Time (PT)
Partial Thromboplastin time (PTT)
Bleeding time and clotting time
MP (Malaria Parasite)
Reticulocyte count
Absolute Eosinophil count
Blood smear study
Blood smear parasites
Sickle cell preparation
Complete Haemogram
Hb Electrophoresis
CLINICAL PATHOLOGY
Urine routine analysis
Motion routine analysis
Mantoux test
Occult blood
CLINICAL BIOCHEMISTRY
Blood glucose
Urea
Creatinine
Cholesterol
HDL Cholesterol
Triaceyl Glycerol (Triglycerides)
SPECIAL INVESTIGATIONS OFFERED IN CLIMICAL BIOCHEMISTRY
Liver Function Tests
Serum AST
Serum Bilirubin (Total and direct)
Total protein
Albumin
Globulin
Serum Ceruloplasmin
Electrophoresis for serum/ urine/protein
Electrophoresis for serum/ Lipoprotein
Blood Pyruvate and Lactate
Serum Angiotensin Converting Enzyme
Blood Glucose 6 phosphate Dehydrogenase
Serum Lactate dehydrogenase
SerumVitamin A (HPLC)
Paper chromatography for Galactose/any sugars
Paper chromatography for Ornithine / amino acids
Amino acid profile, Plasma (HPLC)
Amino aicd profile, Urine (HPLC)
Serum amylase
Plasma Homocystine – (ELISA)
Serum Thyroid profile: T3, T4, TSH
Serum Vitamin E (HPLC)
Plasma vitamin C
Blood Glutathione
Blood Glutathione Peroxidase
Blood Superoxide dismutase
Blood Thiobarbituric Acid Reactive Substances
HbA1C
Microalbuminuria
Minerals : Copper / Zinc / Selenium / Iron
Instructions to patients for collection of clinical specimens for Biochemical tests

01. Fasting Specimen: For all “Fasting Specimen” collection the patient is instructed to have dinner the previous night not later than 9 p.m. (the range for fasting is 8 to 12 hours) and following this not to eat or drink except water till he reports to the laboratory.

02. Paper-chromatography for Ornithine/any amino acid [specify] – Fasting sample both blood 2 ml (By syringe collection) in ACD and 5 ml urine.

03. Paper-chromatography for any sugar e.g. Galactose/fructose- One Heparin vacutainer/5 ml urine (fasting condition)

ELISA for Homocystine- One EDTA vacutainer (2ml) in fasting condition plasma to be separate and send immediately..
Aminoacid profile, HPLC-plasma 2ml in ACD vial (By syringe collection) in fasting condition
Aminoacid profile,HPLC-urine in fasting condition
Microalbumin – Sterile urine in fasting condition.
04. Random Specimen
Glycosylated Haemoglobin - One vacutainer 2ml of EDTA
One Plain 4ml vacutainer for ceruloplansim , uric acid, AST, Serum Lactate dehyrdogenase
Trace elements 4 ml plain and Heparin to be collected
For Serum Iron, TIBC and Percentage saturation testing the patient are instructed to report for blood collection at the collection area between 9 am and 11 am without fasting.
One 4ml heparin for serum Amylase.

05. Instructions to Patients coming for serum Vitamin A and E testing: The patient should not take any Vitamin tonics/ Leafy Vegetables for 2 days prior to the test. 5ml plain sample to be collected and separate the serum and send with patient history.

06. Instructions to Patients coming for serum Vitamin C testing. Any medications are taken by patient to be asked 5 ml heparin to be collected.

07. Instructions to the patients who have undergone FFA (Fundus Fluorescein Angiography)
In the above mentioned conditions the patients are instructed not to give blood for 48 hours after the FFA for the following test, Angiotensin Converting Enzyme (ACE) and Plasma Homocystine.

08. Electrophoresis for Protein and Lipo protein Serum/urine/CSF:
4ml plain vacutainer tube to be collected and sent on the same day to the laboratory..

09. Antioxidants along with the package Vitamin also will be done: 3EDTA, 2 plain and 2 Heparin to be collected send the sample without separation. Plain sample alone to be separate for vitamin analysis..

10. Minerals: Copper/Zinc/Selenium/Iron. 4ml palin sample to be collected.

11. Blood pyruvate and lactate: 2 ml whole blood to be added in 4ml of perchloric acid for pyruvate and lactate . Separate the supernatant and send it.

12. Urine Inborn errors and metabolism need.Fasting Specimen: For all “Fasting Specimen” collection the patient is instructed to have dinner the previous night not later than 9 p.m. (the range for fasting is 8 to 12 hours) and following this not to eat or drink except water till he reports to the laboratory.