Primary BRPE cells		Dividing epithelial cells

Cells after 1st passage		Actin

Vimentin		Cytokeratin 8.13

Cytokeratin – WB , showing a 46 KD band at passages 1-5 as indicated by the lanes 1-5
FITC conjugated Ab /Counter stain by Evans blue

ARPE – 19 cells (ATCC)

ARPE 19 confluent layer		Cytokeratin 8.13 Positive

Cytplasmic postivity of FITC labelled (green) cytokeratin 8.13 characteristic of RPE

Figure 2 : Primary culture of Bovine retinal capillary endothelial cells (BREC) cells and the markers of BREC cells.

A
B		C

A: Confluent BREC cells after isolation with CD31 coated dynabeads,
B: Factor VIII positivity in BREC cells green fluorescence cytoplasmic,
C: VE cadherin positivity in BREC cells green fluorescence membrane

Figure 3 : Primary culture of Bovine retinal capillay pericytes (BRP )cells and the markers

A
B		C

A: Confluent BRP cells on day 12,
B: NG2 positive BRP cells green fluorescence membrane positivity ,
C: Actin filaments stained brown shown by immunohistochemistry in BRP cells.

A
B		C

Fig. 1: 3T3-L1 fibroblast cell line passage 11: Preadipocyte.
Fig. 2: Differentiated adipocyte cell line with oil droplets (10X).
Fig. 3: Oil red O stain for lipid droplets in adipocytes (20 X)

NCCS Pune

CHO-K1 NCCS PUNE